ABSTRACT
Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor [PRGF] at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts [hPLF] cultured on Hypro-Sorb F, Pericardium and Tutodent resorbable membranes coated with PRGF
Methods: This experimental study was conducted on four resorbable membranes namely Hypro- Sorb F, Pericardium, Tutodent and Vicryl. Fibroblast cells isolated from the periodontal ligament of premolar tooth were passaged three times and 10[5]cells/ cm[2] were cultured on membranes coated with PRGF. After 72 hours, the cells were evaluated in terms of biocompatibility and alkaline phosphatase [ALP] activity. Statistical analysis was carried out using one-way ANOVA
Results: PRGF increased cell adhesion and Tutodent membrane coated with PRGF showed the highest cell adhesion compared with Hypro-Sorb F and Pericardium membranes [p=0.005]
Conclusion: PRGF increases cell viability and ALP activity of cells on biomembranes. PRGF treatment increases the adhesion of fibroblast cells to these membranes
ABSTRACT
Considering the importance to determine the reasons for the higher occurrence of congenital hypothyroidism [CH] in Iran, in this study we report the prevalence of permanent CH [PCH] in Isfahan province 7 years after initiation of CH screening program in Isfahan. In this cross sectional study, children with a primary diagnosis of CH studied. They clinically examined and their medical files were reviewed by a pediatric endocrinologist. Considering screening and follow-up lab data, radiologic findings and the decision of pediatric endocrinologists the final diagnosis of PCH was determined. A total of 464,648 neonates screened in Isfahan province. The coverage percent of the CH screening and recall rate was 98.9% and 2.1%, respectively. A total of 1990 neonates were diagnosed with primary CH. PCH was diagnosed in 410 neonates. The prevalence of PCH and transient CH [TCH] was 1 in 1133 and 1 in 294 live births. The most common etiology of CH was thyroid dyshormonogenesis. Though the prevalence of PCH is high, but the higher prevalence of CH in Isfahan is commonly due to cases with TCH. Hence, the necessity of determining new strategies for earlier diagnosis of patients with TCH is recommended
Subject(s)
Humans , Female , Male , Congenital Hypothyroidism/etiology , Prevalence , Cross-Sectional StudiesABSTRACT
Lipoid congenital adrenal hyperplasia [lipoid CAH], a rare disorder of steroid biosynthesis, is the most severe form of CAH. In this disorder the synthesis of glucocorticoids, mineralocorticoids and sex steroids is impaired which result in adrenal failure, severe salt wasting crisis and hyperpigmentation in phenotypical female infants irrespective of genetic sex. In this report, we presented a 28-day-old phenotypic female infant, which referred with lethargy, failure to thrive and electrolyte abnormalities. Considering the clinical and biochemical findings, lipoid CAH was diagnosed and replacement therapy with standard doses of glucocorticoid and mineralocorticoid and sodium chloride was initiated. During follow-up, she had good clinical condition, but at 6 years of age, she refers with hypertension and adrenal insufficiency because of arbitrary drug discontinuation by mother. In ultrasonography an abdominal mass [the testicles] was reported. Chromosome study showed 46XY pattern. Orchiectomy was performed. We recommended that in cases with clinical presentation of adrenal insufficiency if there is not the facility to determine the karyotype, repeated ultrasonography perform during follow-up. In addition, investigating the genetic bases of the disorder would help us to determine the pathogenesis of lipoid CAH in our community. It would be helpful in prenatal diagnosis and treatment of the disorder to prevent its related comorbidities